human non small cell lung cancer nsclc cell lines a549  (ATCC)

Journal: Drug Design, Development and Therapy

Article Title: Rhein Alleviates Cisplatin-Induced Acute Kidney Injury via Downregulation of NOX4-COX2/PGFS Signaling Pathway

doi: 10.2147/DDDT.S515409

Figure Lengend Snippet: Rhein reduces CDDP-induced nephrotoxicity without affecting the antitumor efficacy. CDDP (10μg/mL)-treated human non-small-cell lung cancer (NSCLC) A549 cells were incubated with 40 μM Rhein for 24 h. ( A ) The cell viability was determined by the CCK8 analysis (n=6). ( B ) The CDDP-sensitive Lewis lung carcinoma (LLC) cells (2×10 5 ) were inoculated subcutaneously into C57BL/6 mice for 14 days, and the mice were treated with CDDP alone (4 mg/kg, the drugs were administered every other day for 10 days, resulting in an accumulative dose of 20 mg/kg) or combination with Rhein (80mg/kg, the drugs were administered 1 hour after CDDP injection). ( C and D ) The xenograft tumor weights and tumor volume of C57BL/6 mice injected subcutaneously with LLC cells with the treatment of CDDP, Rhein alone or in combination with Rhein (n=5). Data are shown as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001: CDDP vs Control. ## P < 0.01 CDDP+Rhein vs CDDP.

Article Snippet: To determine whether Rhein might affect the anti-tumor activity of CDDP in vitro, CDDP-sensitive human non-small-cell lung cancer (NSCLC) cell line A549 (catalog no. CCL-185, RRID: CVCL_0023; ATCC, Manassas, VA, USA) was exposed to CDDP (10 μg/mL) alone or in combination with Rhein for 24h, cell viability was then measured.

Techniques: Incubation, Injection, Control

Journal: Briefings in bioinformatics

Article Title: Comparative study of the mechanism of natural compounds with similar structures using docking and transcriptome data for improving in silico herbal medicine experimentations.

doi: 10.1093/bib/bbad344

Figure Lengend Snippet: Figure 4. Results of RNA-seq analysis of OA, HG and GA. The A549 cell line was treated with OA, HG, a combination of OA and HG (COH) and GA, and the MOA of each compound was derived using transcriptome data. Gene set enrichment analysis (GSEA) was performed using the gene expression results derived from each compound. (A) Venn diagram is shown using the genes selected as DEGs as a result of RNA-seq analysis of OA, HG, COH and GA. Commonly selected DEGs in OA, HG and COH are highlighted in orange circles. (B) A volcano plot was constructed using gene expression information derived from COH. (C) GSEA results of OA, HG, COH and GA were calculated using the KEGG pathway gene sets and RNA-seq analysis results of OA, HG, COH and GA.

Article Snippet: Human non-small cell lung cancer cell (NSCLC) line A549 (CCL185) was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: RNA Sequencing, Derivative Assay, Gene Expression, Construct